IL-5 anticorps

N° du produit ABIN116079

Cet anticorps anti-IL-5 est un anticorps Lapin Polyclonal détectant IL-5 dans WB, EIA, Func et IHC (p). Adapté pour Humain.

Aperçu rapide pour IL-5 anticorps (ABIN116079)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-5 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-IL-5 anticorps

Specificité: This antibody reacts with Human Interleukin-5. Other species not tested.

Purification: Affinity Chromatography.

Immunogène: Highly pure (>98%) recombinant Human IL-5.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5(0.45 ng/mL), a concentration of 0.05-0.09 μg/mL of this antibody is required. ELISA: To detect hIL-5 by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur IL-5

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: Interleukin-5 / IL5

Sujet: Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF

ID gène: 3567

NCBI Accession: NP_000870

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour